Oasis blue dating
All soil samples were collected under sterile conditions using sterile tools.Recovered samples were stored at −20°C for molecular analysis or at 4°C for isolation.Université de La Manouba, Institut Supérieur de Biotechnologie de Sidi Thabet, LR11ES31 LR Biotechnologie & Valorisation des Bio-Géo Ressources, Biotech Pole Sidi Thabet, 2020 Ariana, Tunisia Received ; Accepted 6 October 2014Academic Editor: Sara Borin Copyright © 2015 Raoudha Ferjani et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The bacterial communities in the soil fractions associated with the root system of date palms cultivated in seven oases in Tunisia were assessed by culture-independent and dependent approaches.
(b) A principal coordinate analysis (PCO), deduced from the 16S r RNA gene-based PCR-DGGE profiles, resumes the diversity of the bacterial communities associated with the date palm root soil fractions.
84.8% of total variation is explained in the presented PCO.
The soil fractions analysed are R: rhizosphere; S: root surrounding soil; and B: bulk soil. E: longitude east; Alt.: altitude; : maximum temperature; Rainfall min: minimum rainfall; Rainfall max: maximum rainfall.
(c) Dist LM analysis to evaluate which are the main geoclimatic variables influencing the structure of the bacterial communities associated with date palm root soil fractions. (d) Box-plot graph represents the quantification of 16S r RNA gene by q PCR.